Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies.
Identifieur interne : 003412 ( Main/Exploration ); précédent : 003411; suivant : 003413Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies.
Auteurs : Rajeev Kumar [États-Unis] ; Charles E. WymanSource :
- Biotechnology progress [ 1520-6033 ]
Descripteurs français
- KwdFr :
- Acides (composition chimique), Biotechnologie (méthodes), Bois (composition chimique), Cellulase (composition chimique), Cellulose (composition chimique), Dioxyde de soufre (composition chimique), Endo-1,4-beta xylanases (composition chimique), Hydrolyse (MeSH), Ressources de production d'énergie (MeSH), bêta-Glucosidase (composition chimique).
- MESH :
- composition chimique : Acides, Bois, Cellulase, Cellulose, Dioxyde de soufre, Endo-1,4-beta xylanases, bêta-Glucosidase.
- méthodes : Biotechnologie.
- Hydrolyse, Ressources de production d'énergie.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Acids, Cellulase, Cellulose, Endo-1,4-beta Xylanases, Sulfur Dioxide, beta-Glucosidase.
- chemistry : Wood.
- methods : Biotechnology.
- Energy-Generating Resources, Hydrolysis.
Abstract
Comparative data is presented on glucose and xylose release for enzymatic hydrolysis of solids produced by pretreatment of poplar wood by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid, flowthrough (FT), lime, and sulfur dioxide (SO(2)) technologies. Sugar solubilization was measured for times of up to 72 h using cellulase supplemented with beta-glucosidase at an activity ratio of 1:2, respectively, at combined protein mass loadings of 5.8-116 mg/g of glucan in poplar wood prior to pretreatment. In addition, the enzyme cocktail was augmented with up to 11.0 g of xylanase protein per gram of cellulase protein at combined cellulase and beta-glucosidase mass loadings of 14.5 and 29.0 mg protein (about 7.5 and 15 FPU, respectively)/g of original potential glucose to evaluate cellulase-xylanase interactions. All pretreated poplar solids required high protein loadings to realize good sugar yields via enzymatic hydrolysis, and performance tended to be better for low pH pretreatments by dilute sulfuric acid and sulfur dioxide, possibly due to higher xylose removal. Glucose release increased nearly linearly with residual xylose removal by enzymes for all pretreatments, xylanase leverage on glucan removal decreased at high cellulase loadings. Washing the solids improved digestion for all pretreatments and was particularly beneficial for controlled pH pretreatment. Furthermore, incubation of pretreated solids with BSA, Tween 20, or PEG6000 prior to adding enzymes enhanced yields, but the effectiveness of these additives varied with the type of pretreatment.
DOI: 10.1002/btpr.102
PubMed: 19301243
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies.</title><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, NH 03755, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Thayer School of Engineering, Dartmouth College, NH 03755</wicri:regionArea><placeName><region type="state">New Hampshire</region></placeName></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009 Mar-Apr</date><idno type="RBID">pubmed:19301243</idno><idno type="pmid">19301243</idno><idno type="doi">10.1002/btpr.102</idno><idno type="wicri:Area/Main/Corpus">003610</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003610</idno><idno type="wicri:Area/Main/Curation">003610</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003610</idno><idno type="wicri:Area/Main/Exploration">003610</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies.</title><author><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name><affiliation wicri:level="2"><nlm:affiliation>Thayer School of Engineering, Dartmouth College, NH 03755, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Thayer School of Engineering, Dartmouth College, NH 03755</wicri:regionArea><placeName><region type="state">New Hampshire</region></placeName></affiliation></author><author><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></author></analytic><series><title level="j">Biotechnology progress</title><idno type="eISSN">1520-6033</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acids (chemistry)</term><term>Biotechnology (methods)</term><term>Cellulase (chemistry)</term><term>Cellulose (chemistry)</term><term>Endo-1,4-beta Xylanases (chemistry)</term><term>Energy-Generating Resources (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Sulfur Dioxide (chemistry)</term><term>Wood (chemistry)</term><term>beta-Glucosidase (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides (composition chimique)</term><term>Biotechnologie (méthodes)</term><term>Bois (composition chimique)</term><term>Cellulase (composition chimique)</term><term>Cellulose (composition chimique)</term><term>Dioxyde de soufre (composition chimique)</term><term>Endo-1,4-beta xylanases (composition chimique)</term><term>Hydrolyse (MeSH)</term><term>Ressources de production d'énergie (MeSH)</term><term>bêta-Glucosidase (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acids</term><term>Cellulase</term><term>Cellulose</term><term>Endo-1,4-beta Xylanases</term><term>Sulfur Dioxide</term><term>beta-Glucosidase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Wood</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Acides</term><term>Bois</term><term>Cellulase</term><term>Cellulose</term><term>Dioxyde de soufre</term><term>Endo-1,4-beta xylanases</term><term>bêta-Glucosidase</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biotechnology</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Biotechnologie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Energy-Generating Resources</term><term>Hydrolysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Hydrolyse</term><term>Ressources de production d'énergie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Comparative data is presented on glucose and xylose release for enzymatic hydrolysis of solids produced by pretreatment of poplar wood by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid, flowthrough (FT), lime, and sulfur dioxide (SO(2)) technologies. Sugar solubilization was measured for times of up to 72 h using cellulase supplemented with beta-glucosidase at an activity ratio of 1:2, respectively, at combined protein mass loadings of 5.8-116 mg/g of glucan in poplar wood prior to pretreatment. In addition, the enzyme cocktail was augmented with up to 11.0 g of xylanase protein per gram of cellulase protein at combined cellulase and beta-glucosidase mass loadings of 14.5 and 29.0 mg protein (about 7.5 and 15 FPU, respectively)/g of original potential glucose to evaluate cellulase-xylanase interactions. All pretreated poplar solids required high protein loadings to realize good sugar yields via enzymatic hydrolysis, and performance tended to be better for low pH pretreatments by dilute sulfuric acid and sulfur dioxide, possibly due to higher xylose removal. Glucose release increased nearly linearly with residual xylose removal by enzymes for all pretreatments, xylanase leverage on glucan removal decreased at high cellulase loadings. Washing the solids improved digestion for all pretreatments and was particularly beneficial for controlled pH pretreatment. Furthermore, incubation of pretreated solids with BSA, Tween 20, or PEG6000 prior to adding enzymes enhanced yields, but the effectiveness of these additives varied with the type of pretreatment.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19301243</PMID><DateCompleted><Year>2009</Year><Month>06</Month><Day>03</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1520-6033</ISSN><JournalIssue CitedMedium="Internet"><Volume>25</Volume><Issue>2</Issue><PubDate><MedlineDate>2009 Mar-Apr</MedlineDate></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol Prog</ISOAbbreviation></Journal><ArticleTitle>Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies.</ArticleTitle><Pagination><MedlinePgn>302-14</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/btpr.102</ELocationID><Abstract><AbstractText>Comparative data is presented on glucose and xylose release for enzymatic hydrolysis of solids produced by pretreatment of poplar wood by ammonia fiber expansion (AFEX), ammonia recycled percolation (ARP), controlled pH, dilute acid, flowthrough (FT), lime, and sulfur dioxide (SO(2)) technologies. Sugar solubilization was measured for times of up to 72 h using cellulase supplemented with beta-glucosidase at an activity ratio of 1:2, respectively, at combined protein mass loadings of 5.8-116 mg/g of glucan in poplar wood prior to pretreatment. In addition, the enzyme cocktail was augmented with up to 11.0 g of xylanase protein per gram of cellulase protein at combined cellulase and beta-glucosidase mass loadings of 14.5 and 29.0 mg protein (about 7.5 and 15 FPU, respectively)/g of original potential glucose to evaluate cellulase-xylanase interactions. All pretreated poplar solids required high protein loadings to realize good sugar yields via enzymatic hydrolysis, and performance tended to be better for low pH pretreatments by dilute sulfuric acid and sulfur dioxide, possibly due to higher xylose removal. Glucose release increased nearly linearly with residual xylose removal by enzymes for all pretreatments, xylanase leverage on glucan removal decreased at high cellulase loadings. Washing the solids improved digestion for all pretreatments and was particularly beneficial for controlled pH pretreatment. Furthermore, incubation of pretreated solids with BSA, Tween 20, or PEG6000 prior to adding enzymes enhanced yields, but the effectiveness of these additives varied with the type of pretreatment.</AbstractText><CopyrightInformation>(c) 2009 American Institute of Chemical Engineers Biotechnol.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajeev</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Thayer School of Engineering, Dartmouth College, NH 03755, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wyman</LastName><ForeName>Charles E</ForeName><Initials>CE</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000143">Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0UZA3422Q4</RegistryNumber><NameOfSubstance UI="D013458">Sulfur Dioxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.21</RegistryNumber><NameOfSubstance UI="D001617">beta-Glucosidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.8</RegistryNumber><NameOfSubstance UI="D043364">Endo-1,4-beta Xylanases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000143" MajorTopicYN="N">Acids</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D043364" MajorTopicYN="N">Endo-1,4-beta Xylanases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004736" MajorTopicYN="N">Energy-Generating Resources</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013458" MajorTopicYN="N">Sulfur Dioxide</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001617" MajorTopicYN="N">beta-Glucosidase</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>3</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>3</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>6</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19301243</ArticleId><ArticleId IdType="doi">10.1002/btpr.102</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>New Hampshire</li></region></list><tree><noCountry><name sortKey="Wyman, Charles E" sort="Wyman, Charles E" uniqKey="Wyman C" first="Charles E" last="Wyman">Charles E. Wyman</name></noCountry><country name="États-Unis"><region name="New Hampshire"><name sortKey="Kumar, Rajeev" sort="Kumar, Rajeev" uniqKey="Kumar R" first="Rajeev" last="Kumar">Rajeev Kumar</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003412 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003412 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:19301243
|texte= Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:19301243" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |